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ATDC5 Whole Cell Lysate

     
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Additional info
Application Notes Ready-to-use ATDC5 whole cell lysate is prepared as a positive control for separation by SDS-PAGE and subsequent western blot analysis. Heat lysate to 95°C for 5 minutes and rapidly cool. The recommended loading volume per lane is 2~10 ul depending on the size format of your gel.
Format Whole lysate is stored in 1 X SDS 0.3% beta-mercaptoethanol sample buffer.
StorageStore vial at -70°C or colder. For extended storage, aliquot contents to minimize freeze/thaw cycles. Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Research Areas
A novel glucosamine derivative with low cytotoxicity enhances chondrogenic differentiation of ATDC5.
Author : Yao H1,2,3,Xue J1,2,Xie R1,2,Liu S1,2,Wang Y1,2,Song W4,5,Wang DA6,Ren L7,8.
J Mater Sci Mater Med. 2017 Sep 27;28(11):170. doi: 10.1007/s10856-017-5971-y.
28956208
BMP-9 dependent pathways required for the chondrogenic differentiation of pluripotent stem cells.
Author : Cheng A1, Gustafson AR2, Schaner Tooley CE2, Zhang M1.
Differentiation. 2016 Apr 4. pii: S0301-4681(15)30078-5. doi: 10.1016/j.diff.2016.03.005. [Epub ahead of print]
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