- 文献引用 : 2
|Application Notes||Ready-to-use ATDC5 whole cell lysate is prepared as a positive control for separation by SDS-PAGE and subsequent western blot analysis. Heat lysate to 95°C for 5 minutes and rapidly cool. The recommended loading volume per lane is 2~10 ul depending on the size format of your gel.|
|Format||Whole lysate is stored in 1 X SDS 0.3% beta-mercaptoethanol sample buffer.|
|Storage||Store vial at -70°C or colder. For extended storage, aliquot contents to minimize freeze/thaw cycles. Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
Author : Yao H1,2,3,Xue J1,2,Xie R1,2,Liu S1,2,Wang Y1,2,Song W4,5,Wang DA6,Ren L7,8.
J Mater Sci Mater Med. 2017 Sep 27;28(11):170. doi: 10.1007/s10856-017-5971-y.
Author : Cheng A1, Gustafson AR2, Schaner Tooley CE2, Zhang M1.
Differentiation. 2016 Apr 4. pii: S0301-4681(15)30078-5. doi: 10.1016/j.diff.2016.03.005. [Epub ahead of print]
Provided below are standard protocols that you may find useful for product applications.