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|Application Notes||Ready-to-use 293 whole cell lysate is prepared as a positive control for separation by SDS-PAGE and subsequent western blot analysis. Heat lysate to 95°C for 5 minutes and rapidly cool. The recommended loading volume per lane is 2~10 ul depending on the size format of your gel.|
|Format||Whole lysate is stored in 1 X SDS 0.3% beta-mercaptoethanol sample buffer.|
|Storage||Store vial at -70°C or colder. For extended storage, aliquot contents to minimize freeze/thaw cycles. Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
Author : Tang M1,Liu Y2,Zhang QC3,Zhang P4,Wu JK2,Wang JN2,Ruan Y2,Huang Y2.
Oncol Lett. 2018 Sep;16(3):2813-2822. doi: 10.3892/ol.2018.9001. Epub 2018 Jun 21.
Provided below are standard protocols that you may find useful for product applications.