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>   首页   >   产品   >   一抗   >   精选抗体   >   斑马鱼抗体   >   Zebrafish PDHA1 Antibody (Center)   

Zebrafish PDHA1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Zebrafish PDHA1 Antibody (Center) AZb9652c
    All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q6P948
Other Accession P26284, P29804, P35486, Q8HXW9, P08559, A7MB35
Reactivity Zebrafish
Predicted Bovine, Human, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB24303
Calculated MW 43741 Da
Additional info
Gene ID 406702
Other Names PDHE1-A type I; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A, PDHA1;pdha1a;pdha1b
Target/Specificity This Zebrafish PDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZebrafish PDHA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Q6P948
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Research Areas

BACKGROUND

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.

REFERENCES

Glushakova, L.G., et al. Mol. Genet. Metab. 98(3):289-299(2009) Joao Silva, M., et al. Eur. J. Pediatr. 168(1):17-22(2009) Boichard, A., et al. Mol. Genet. Metab. 93(3):323-330(2008)

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