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Vimentin Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Vimentin Antibody AX10005
    All lanes : Anti-VIME Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Vimentin Antibody AX10005
    Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - Vimentin Antibody AX10005
    Vimentin Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.
  • 3 - Vimentin Antibody AX10005
    Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
  • 3 - Vimentin Antibody AX10005
    Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
  • 3 - Vimentin Antibody AX10005
    Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, FC, IHC-P, E
Primary Accession P08670
Other Accession NP_003371.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB41034
Additional info
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.
Dilution WB~~1:4000
IHC-P~~1:50
FC~~1:50
IF~~1:50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas

BACKGROUND

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

REFERENCES

Kers, J., et al. Transplantation 90(5):502-509(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Korita, P.V., et al. Anticancer Res. 30(6):2279-2285(2010)
Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press :
Li, M., et al. J. Exp. Clin. Cancer Res. 29, 109 (2010) :

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