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Hsp60 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Hsp60 Antibody AW5684
    All lanes : Anti-Hsp60 Antibody at 1:40000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C2C12 whole cell lysate Lane 7: PC-12 whole cell lysate Lane 8: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P10809
Other Accession P31081, Q5ZL72, P18687, Q39727, P63038, Q5NVM5, P63039
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Dog, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB55971
Calculated MW H=61;M=61,27;R=61 KDa
Additional info
Gene ID 3329
Other Names 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Target/Specificity This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from human Hsp60.
Dilution WB~~1:40000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHsp60 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPD1
Synonyms HSP60
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Cellular Location Mitochondrion matrix.

BACKGROUND

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

REFERENCES

Jindal S.,et al.Mol. Cell. Biol. 9:2279-2283(1989).
Venner T.J.,et al.DNA Cell Biol. 9:545-552(1990).
Hansen J.J.,et al.Hum. Genet. 112:71-77(2003).
Tan J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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