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beta Tubulin

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - beta Tubulin AW5683
    All lanes : Anti-beta Tubulin Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C2C12 whole cell lysate Lane 7: PC-12 whole cell lysate Lane 8: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P99024
Other Accession Q17299, P12456, P09203, Q24560, Q9YHC3, Q27U48, O17449, P36221, Q6EVK8, Q13885, Q4R5B3, Q7TMM9, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P52275, P32882, P83130, P61858, P61857, P13602, Q2T9S0, P09206, Q13509, Q60HC2, Q9ERD7, Q4QRB4, Q3ZBU7, P04350, Q4R4X8, Q9D6F9, Q3MHM5, P68371, P86221, P68
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Monkey, Dog, Sheep, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB55943
Calculated MW M=50 KDa
Additional info
Gene ID 22154
Other Names Tubulin beta-5 chain, Tubb5
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.
Dilution WB~~1:4000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta Tubulin is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tubb5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons.

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Wang D.,et al.J. Cell Biol. 103:1903-1910(1986).
Carninci P.,et al.Science 309:1559-1563(2005).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).

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