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beta II Tubulin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - beta II Tubulin Antibody AW5682
    All lanes : Anti-beta II Tubulin at1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: human brain lysate Lane 4: human Kidney lysate Lane 5: NIH/3T3 whole cell lysate Lane 5: mouse lung lysate Lane 5: PC12 whole cell lysate Lane 5: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q7TMM9
Other Accession P09203, Q9NFZ7, Q13885, Q4R5B3, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P32882, P13602, Q9NFZ5, P30156, P20802, O59837, P02554
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Monkey, Dog, Sheep, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW M=50;H=50;R=50 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 22151
Antigen Region 30-46
Other Names Tubulin beta-2A chain, Tubb2a, Tubb2
Dilution WB~~1:2000
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-46 amino acids from human.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta II Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tubb2a
Synonyms Tubb2
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Cellular Location Cytoplasm, cytoskeleton.

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

REFERENCES

Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Janke C.,et al.Science 308:1758-1762(2005).
Rogowski K.,et al.Cell 137:1076-1087(2009).
Yoshida K.,et al.Biochem. Biophys. Res. Commun. 389:506-511(2009).

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