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Myc Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - Myc Tag Antibody AW5677
    All lanes : Anti-Myc Tag Antibody at 1:1000 dilution Lane 1: 293T/17 transfected with 12tag lysate (1ug) Lane 2: Non-transfected 293T/17 lysate (1ug) Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Mouse
Clonality Monoclonal
Calculated MW 45-50KD KDa
Isotype Mouse IgG1 
Antigen Source HUMAN
Additional Information
Antigen Region N/A
Other Names Tag from c-Myc protein
Dilution WB~~1:1000
Target/Specificity KLH conjugated synthetic peptide encoding c-Myc tag was used as antigen.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyc Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

REFERENCES

Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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