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HIS Tag Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - HIS Tag Antibody AW5672
    All lanes : Anti-HIS Tag at 1:1000 dilution (upper) or GAPDH (lower) Lane 1: 293T/17 transfected with 12tag lysate (10ug) Lane 2: Non-transfected 293T/17 lysate (10ug) Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Mouse
Clonality Monoclonal
Calculated MW 45-50KD KDa
Isotype Mouse IgG1 
Antigen Source HUMAN
Additional Information
Antigen Region NA
Dilution WB~~1:1000
Target/Specificity Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

REFERENCES

Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.

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