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GST Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - GST Tag Antibody AW5668
    Anti-GST Tag Antibody at1:1000 dilution + 12tag recombinant protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GST Tag Antibody AW5668
    All lanes : Anti-GST Tag Antibody at1:1000 dilution Lane 1: 293T/17 transfected with 12tag lysate(10ug) Lane 2: Non-transfected 293T/17 lysate(10ug) Lane 3: 293T/17 transfected with 12tag lysate(1ug) Lane 4: Non-transfected 293T/17 lysate(1ug) Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 9AT106
Calculated MW 45-50KD KDa
Additional info
Other Names Glutathione S-transferase
Target/Specificity Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGST Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information

BACKGROUND

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

REFERENCES

Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31.
Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
Toye, B. et al., (1990) Infect. Immun. 58, 3909.
Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262

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