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HA Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HA Tag Antibody AW5664
    All lanes : Anti-HA Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower) Lane 1: 293T/17 transfected with 12tag lysate (10ug) Lane 2: Non-transfected 293T/17 lysate (10ug) Lane 3: 12tag recombinant protein lysate (0. 1ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB56168
Calculated MW 45-50 KDa
Additional info
Other Names Glutathione S-transferase
Target/Specificity KLH conjugated synthetic peptide encoding HA tag was used as antigen.
Dilution WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

REFERENCES

Marco. et.al., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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