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GARS Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - GARS Antibody AW5653
    All lanes : Anti-GARS Antibody at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Ramos whole cell lysate Lane 4: PC-3 whole cell lysate Lane 4: mouse brain lysate Lysates/proteins at 15 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - GARS Antibody AW5653
    Overlay histogram showing Hela cells stained with AW5653(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5653, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB
Primary Accession P41250
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=83;M=82;R=72 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 2617
Antigen Region 15-305
Other Names Glycine--tRNA ligase, 6.1.1.14, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
Dilution WB~~1:1000
FC~~1:25
Target/Specificity This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GARS
Function Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Cellular Location Cytoplasm. Mitochondrion
Tissue Location Widely expressed, including brain and spinal cord.

BACKGROUND

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.

REFERENCES

Shiba K.,et al.J. Biol. Chem. 269:30049-30055(1994).
Williams J.H.,et al.Nucleic Acids Res. 23:1307-1310(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
Ge Q.,et al.J. Biol. Chem. 269:28790-28797(1994).

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