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EEF1E1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - EEF1E1 Antibody AW5638
    All lanes : Anti-EEF1E1 Antibody at1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - EEF1E1 Antibody AW5638
    Overlay histogram showing Hela cells stained with AW5638 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5638, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 3 - EEF1E1 Antibody AW5638
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AW5638 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF
Primary Accession O43324
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,κ
Clone Names 1494CT639.147.31
Calculated MW H=20,16;M=20 KDa
Additional info
Gene ID 9521
Other Names Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18, EEF1E1, AIMP3, P18
Target/Specificity This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.
Dilution WB~~1:2000
FC~~1:25
IF~~1:25
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1E1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1E1
Synonyms AIMP3, P18
Function Positive modulator of ATM response to DNA damage.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage
Tissue Location Down-regulated in various cancer tissues.

BACKGROUND

Positive modulator of ATM response to DNA damage.

REFERENCES

Motegi H.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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