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ENASE Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ENASE Antibody  (Center) AW5630
    All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ENASE Antibody  (Center) AW5630
    ENASE Antibody (Center) (Cat. #AW5630) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ENASE Antibody  (Center) AW5630
    ENASE Antibody (Center) (Cat. #AW5630) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC
Primary Accession Q8NFI3
Other Accession Q8BX80
Reactivity Human, Mouse
Predicted Dog
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB28722
Calculated MW H=84,43;M=83 KDa
Additional info
Gene ID 64772
Other Names Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
Target/Specificity This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
Dilution WB~~0.25
IHC-P~~1:50~100
FC~~1:10~50
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENASE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENGASE
Function Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Cellular Location Cytoplasm, cytosol.
Tissue Location Widely expressed. Expressed at higher level in thymus and spleen.

BACKGROUND

Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.

REFERENCES

Park, K., et al. Diabetes 59(7):1845-1850(2010)
Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
Chantret, I., et al. PLoS ONE 5 (7), E11734 (2010) :
Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)
Ortutay, Z., et al. Arthritis Rheum. 48(8):2163-2172(2003)

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