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ALDOC Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
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  • 4 - ALDOC Antibody (C-term) AW5623
    Overlay histogram showing HL-60 cells stained with AW5621(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5621, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG3 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ALDOC Antibody (C-term) AW5623
    All lanes : Anti-ALDOC Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: rat brain lysate Lane 4: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P09972
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=39;M=39;R=39 KDa
Isotype IgG3
Antigen Source HUMAN
Additional Information
Gene ID 230
Antigen Region 1-364
Other Names Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
Dilution FC~~1:25
WB~~1:2000
Target/Specificity Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDOC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDOC
Synonyms ALDC

REFERENCES

Rottmann W.H., et al. Biochimie 69:137-145(1987).
Buono P., et al. Nucleic Acids Res. 16:4733-4733(1988).
Buono P., et al. Eur. J. Biochem. 192:805-811(1990).
Yu W., et al. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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