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NDUFV1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NDUFV1 Antibody (Center) AW5622
    All lanes : Anti-NDUFV1 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse kidney lysate Lane 5: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P49821
Other Accession P25708, Q54I90, Q0MQI5, Q8HXQ9, Q91YT0, Q0MQI6, Q0MQI4
Reactivity Human, Mouse
Predicted Human, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW H=51,50;M=51 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4723
Antigen Region 194-226
Other Names NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial, Complex I-51kD, CI-51kD, NADH dehydrogenase flavoprotein 1, NADH-ubiquinone oxidoreductase 51 kDa subunit, NDUFV1, UQOR1
Dilution WB~~0.25
Target/Specificity This NDUFV1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 194-226 amino acids from the Central region of human NDUFV1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFV1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFV1
Synonyms UQOR1
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

BACKGROUND

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

REFERENCES

de Coo R.F.M.,et al.Mamm. Genome 10:49-53(1999).
Schuelke M.,et al.Biochem. Biophys. Res. Commun. 245:599-606(1998).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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