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GMCL1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GMCL1 Antibody (Center) AW5607
    All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 2 - GMCL1 Antibody (Center) AW5607
    AW5625 staining GMCL1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC, WB
Primary Accession Q96IK5
Other Accession Q8NEA9
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=59,H=60[Q8NEA9];M=60 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 64395
Antigen Region 281-315
Other Names Germ cell-less protein-like 1, GMCL1, GCL
Dilution WB~~0.25
IHC~~1:25
Target/Specificity This GMCL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-315 amino acids from the Central region of human GMCL1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGMCL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GMCL1
Synonyms GCL
Function Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).
Cellular Location Nucleus matrix.

BACKGROUND

Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Holaska J.M.,et al.J. Biol. Chem. 278:6969-6975(2003).

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