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PPM1H Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PPM1H Antibody (Center) AW5604
    All lanes : Anti-PPM1H Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse spleen lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PPM1H Antibody (Center) AW5604
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PPM1H Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - PPM1H Antibody (Center) AW5604
    PPM1H Antibody (Center)(Cat. #AW5604) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC
Primary Accession Q9ULR3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB21341
Calculated MW H=56;M=56,52;R=56 KDa
Additional info
Gene ID 57460
Other Names Protein phosphatase 1H, PPM1H, ARHCL1, KIAA1157, URCC2
Target/Specificity This PPM1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human PPM1H.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPM1H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPM1H
Synonyms ARHCL1, KIAA1157, URCC2
Function Dephosphorylates CDKN1B at 'Thr-187', thus removing a signal for proteasomal degradation.
Cellular Location Nucleus. Cytoplasm

REFERENCES

Sugiura,T., et.al., Cancer Biol. Ther. 7 (2), 285-292 (2008)
Labes,M., et.al., Mol. Cell. Neurosci. 12 (1-2), 29-47 (1998)

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