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HSPA9 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HSPA9 Antibody (Center) AW5599
    All lanes : Anti-HSPA9 Antibody (Center) at 1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: L929 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P38646
Other Accession Q3ZCH0, O35501, P38647, Q5R511, P48721
Reactivity Human, Mouse
Predicted Human, Dog, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=74;M=73;R=74 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3313
Antigen Region 273-301
Other Names Stress-70 protein, mitochondrial, 75 kDa glucose-regulated protein, GRP-75, Heat shock 70 kDa protein 9, Mortalin, MOT, Peptide-binding protein 74, PBP74, HSPA9, GRP75, HSPA9B, mt-HSP70
Dilution WB~~1:2000
Target/Specificity This HSPA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the Central region of human HSPA9.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA9
Synonyms GRP75, HSPA9B, mt-HSP70
Function Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Cellular Location Mitochondrion. Nucleus, nucleolus

BACKGROUND

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

REFERENCES

Li, Y., et al. Environ. Health Perspect. 118(7):936-942(2010)
Luo, W.I., et al. Protein Expr. Purif. 72(1):75-81(2010)
Goswami, A.V., et al. J. Biol. Chem. 285(25):19472-19482(2010)
Iosefson, O., et al. FEBS Lett. 584(6):1080-1084(2010)
Rikova, K., et al. Cell 131(6):1190-1203(2007)

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