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FBXO11 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FBXO11 Antibody (N-term) AW5592
    All lanes : Anti-FBXO11 Antibody (N-term) at 1:1000 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q86XK2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=104,23,107 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 80204
Antigen Region 55-83
Other Names F-box only protein 11, Protein arginine N-methyltransferase 9, Vitiligo-associated protein 1, VIT-1, FBXO11, FBX11, PRMT9, VIT1
Dilution WB~~1:1000
Target/Specificity This FBXO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the N-terminal region of human FBXO11.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFBXO11 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FBXO11
Synonyms FBX11, PRMT9, VIT1
Function Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF- beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of p53/TP53.
Cellular Location Nucleus. Chromosome.
Tissue Location Isoform 5 is expressed in keratinocytes, fibroblasts and melanocytes.
Research Areas

BACKGROUND

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

REFERENCES

Guan, C., et al. Int. J. Mol. Med. 26(1):57-65(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Guan, C.P., et al. Zhonghua Yi Xue Za Zhi 90(16):1126-1130(2010)
Abida, W.M., et al. J. Biol. Chem. 282(3):1797-1804(2007)
Segade, F., et al. Arch. Otolaryngol. Head Neck Surg. 132(7):729-733(2006)

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