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HDGF Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HDGF Antibody (C-term) AW5589
    All lanes : Anti-HDGF Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - HDGF Antibody (C-term) AW5589
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term) (Cat.#AW5589), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - HDGF Antibody (C-term) AW5589
    Confocal immunofluorescent analysis of HDGF Antibody (C-term)(Cat#AW5589) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P51858
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=27,26,28;M=26 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3068
Antigen Region 171-197
Other Names Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
Dilution WB~~1:2000
IHC-P~~1:10~50
IF~~1:10~50
Target/Specificity This HDGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-197 amino acids from the C-terminal region of human HDGF.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHDGF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HDGF
Synonyms HMG1L2
Function Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.

REFERENCES

Thakar,K., FEBS J. 275 (7), 1411-1426 (2008)
Chang,K.C.,Int. J. Cancer 121 (5), 1059-1065 (2007)
Yamamoto,S., Ann. Surg. Oncol. 14 (7), 2141-2149 (2007)
Uyama,H., Clin. Cancer Res. 12 (20 PT 1), 6043-6048 (2006)

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