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PSME2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PSME2 Antibody (C-term) AW5586
    All lanes : Anti-PSME2 Antibody (C-term) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PSME2 Antibody (C-term) AW5586
    Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - PSME2 Antibody (C-term) AW5586
    PSME2 Antibody (C-term) (Cat. #AW5586) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession Q9UL46
Other Accession Q5E9G3, P97372, Q63798
Reactivity Human, Mouse
Predicted Human, Dog, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=27;M=27;R=27 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5721
Antigen Region 210-239
Other Names Proteasome activator complex subunit 2, 11S regulator complex subunit beta, REG-beta, Activator of multicatalytic protease subunit 2, Proteasome activator 28 subunit beta, PA28b, PA28beta, PSME2
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This PSME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the C-terminal region of human PSME2.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSME2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSME2
Function Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Research Areas

BACKGROUND

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 is the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon.

REFERENCES

Conticello,S.G., Curr. Biol. 13 (22), 2009-2013 (2003)

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