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PEF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PEF1 Antibody (Center) AW5584
    All lanes : Anti-PEF1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9UBV8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=30;M=29;R=30 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 553115
Antigen Region 95-121
Other Names Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32
Dilution WB~~1:1000
Target/Specificity This PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human PEF1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PEF1
Synonyms ABP32
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+)
Research Areas

BACKGROUND

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.

REFERENCES

Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hansen, C., et al. FEBS Lett. 545 (2-3), 151-154 (2003) :
Satoh, H., et al. Biochim. Biophys. Acta 1600 (1-2), 61-67 (2002) :
Kitaura, Y., et al. Arch. Biochem. Biophys. 399(1):12-18(2002)
Kitaura, Y., et al. J. Biol. Chem. 276(17):14053-14058(2001)

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