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PEF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PEF1 Antibody (Center) AW5584
    All lanes : Anti-PEF1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9UBV8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=30;M=29;R=30 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 553115
Antigen Region 95-121
Other Names Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32
Dilution WB~~1:1000
Target/Specificity This PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human PEF1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPEF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PEF1 (HGNC:30009)
Synonyms ABP32
Function Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
Cellular Location Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q641Z8}. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+) (PubMed:11278427). Localizes to endoplasmic reticulum exit site (ERES) (By similarity) {ECO:0000250|UniProtKB:Q641Z8, ECO:0000269|PubMed:11278427}
Research Areas

BACKGROUND

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.

REFERENCES

Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hansen, C., et al. FEBS Lett. 545 (2-3), 151-154 (2003) :
Satoh, H., et al. Biochim. Biophys. Acta 1600 (1-2), 61-67 (2002) :
Kitaura, Y., et al. Arch. Biochem. Biophys. 399(1):12-18(2002)
Kitaura, Y., et al. J. Biol. Chem. 276(17):14053-14058(2001)

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