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PCBP1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PCBP1 Antibody (Center) AW5581
    All lanes : Anti-PCBP1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15365
Other Accession Q5E9A3, P60335, O19048
Reactivity Human
Predicted Human, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW H=37,M=37 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5093
Antigen Region 188-217
Other Names Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB23, PCBP1
Dilution WB~~1:1000
Target/Specificity This PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human PCBP1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCBP1
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
Cellular Location Nucleus. Cytoplasm. Note=Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm
Tissue Location Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands

BACKGROUND

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

REFERENCES

Cloke, B., et al. Endocrinology 151(8):3954-3964(2010)
Wang, H., et al. Cancer Cell 18(1):52-62(2010)
Zhang, T., et al. Mol. Cancer 9, 72 (2010) :
Waggoner, S.A., et al. J. Biol. Chem. 284(14):9039-9049(2009)
Huo, L.R., et al. Biochim. Biophys. Acta 1784(11):1524-1533(2008)

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