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PDIA3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PDIA3 Antibody (C-term) AW5556
    All lanes : Anti-PDIA3 Antibody (C-term) at 1:1000 dilution Lane 1: A2058 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T/17 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PDIA3 Antibody (C-term) AW5556
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - PDIA3 Antibody (C-term) AW5556
    PDIA3 Antibody (C-term) (Cat. #AW5556) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P30101
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=57;M=57;R=57 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2923
Antigen Region 446-475
Other Names Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3, ERP57, ERP60, GRP58
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human PDIA3.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDIA3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDIA3
Synonyms ERP57, ERP60, GRP58
Cellular Location Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Detected in the flagellum and head region of spermatozoa (at protein level).
Research Areas

BACKGROUND

PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

REFERENCES

Vigneron,N., et.al., Eur. J. Immunol. 39 (9), 2371-2376 (2009)
Xu,D.,et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L44-L51 (2009)

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