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TMED10 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TMED10 Antibody (C-term) AW5551
    All lanes : Anti-TMED10 Antibody (C-term) at 1:1000 dilution Lane 1: PC-12 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - TMED10 Antibody (C-term) AW5551
    All lanes : Anti-TMED10 Antibody (C-term) at 1:1000 dilution Lane 1: PC-12 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P49755
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=25;M=25,11;R=25(20kd左右) KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 10972
Antigen Region 156-185
Other Names Transmembrane emp24 domain-containing protein 10, 21 kDa transmembrane-trafficking protein, S31III125, S31I125, Tmp-21-I, Transmembrane protein Tmp21, p23, p24 family protein delta-1, p24delta1, p24delta, TMED10, TMP21
Dilution WB~~1:1000
Target/Specificity This TMED10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the C-terminal region of human TMED10.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTMED10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMED10
Synonyms TMP21
Function Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
Cellular Location Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Melanosome. Endoplasmic reticulum membrane; Single-pass type I membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type I membrane protein. Cell membrane. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV Cycles between compartments of the early secretatory pathway
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8.

REFERENCES

Wang, H., et al. Mol. Biol. Cell 21(8):1398-1408(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
Pardossi-Piquard, R., et al. J. Biol. Chem. 284(42):28634-28641(2009)
Soranzo, N., et al. PLoS Genet. 5 (4), E1000445 (2009) :
Liu, S., et al. Eur. J. Neurosci. 28(10):1980-1988(2008)

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