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GTF2E1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GTF2E1 Antibody (N-term) AW5548
    All lanes : Anti-GTF2E1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - GTF2E1 Antibody (N-term) AW5548
    GTF2E1 Antibody (N-term) (AW5548)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GTF2E1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P29083
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=49;M=50 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2960
Antigen Region 93-121
Other Names General transcription factor IIE subunit 1, General transcription factor IIE 56 kDa subunit, Transcription initiation factor IIE subunit alpha, TFIIE-alpha, GTF2E1, TF2E1
Dilution WB~~1:1000
IHC-P~~1:10~50
Target/Specificity This GTF2E1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-121 amino acids from the N-terminal region of human GTF2E1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGTF2E1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GTF2E1
Synonyms TF2E1
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Cellular Location Nucleus.

BACKGROUND

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

REFERENCES

Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Okuda, M., et al. EMBO J. 27(7):1161-1171(2008)
Di Lello, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):106-111(2008)
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Okuda, M., et al. J. Biol. Chem. 279(49):51395-51403(2004)

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