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LDHA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - LDHA Antibody (C-term) AW5540
    All lanes : Anti-LDHA Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: human placenta lysate Lane 4: PC-3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - LDHA Antibody (C-term) AW5540
    Confocal immunofluorescent analysis of LDHA Antibody (C-term)(Cat#AW5540) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 14 - LDHA Antibody (C-term) AW5540
    LDHA Antibody (C-term) (Cat. #AW5540)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDHA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P00338
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=40,30,27,37;M=36;R=36 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3939
Antigen Region 204-232
Other Names L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
Target/Specificity This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLDHA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LDHA
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

REFERENCES

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Koukourakis, M.I., et al. Oncology 77(5):285-292(2009)

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