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Glycerol kinase (GPK2) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Glycerol kinase (GPK2) Antibody (C-term) AW5534
    All lanes : Anti-GPK2/3 Antibody (T502) at 1:1000 dilution Lane 1: human liver lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - Glycerol kinase (GPK2) Antibody (C-term) AW5534
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession Q14410
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=61;M=61 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2712
Antigen Region 487-515
Other Names Glycerol kinase 2, GK 2, Glycerokinase 2, ATP:glycerol 3-phosphotransferase 2, Glycerol kinase, testis specific 2, GK2, GKP2, GKTA
Dilution WB~~1:1000
IHC-P~~1:50~100
Target/Specificity This Glycerol kinase (GPK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human Glycerol kinase (GPK2).
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGlycerol kinase (GPK2) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GK2
Synonyms GKP2, GKTA
Function Key enzyme in the regulation of glycerol uptake and metabolism.
Cellular Location Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=In sperm the majority of the enzyme is bound to mitochondria.
Research Areas

BACKGROUND

The human glycerol kinase gene family consists of at least 3 expressed loci. The GK1 locus on Xp21.3 is the site of mutations (deletions) causing glycerol kinase deficiency. It comprises 19 exons and is probably ancestral to several other genes which, because they are intronless, are suspected of having arisen by reverse transcriptase mediated events. These include 2 genes on chromosome 4. They are expressed as a single mRNA species in testis where expression is at a high level.

REFERENCES

Sargent, C.A., et al., Hum. Mol. Genet. 3(8):1317-1324 (1994).

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