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PECI Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PECI Antibody (C-term) AW5533
    All lanes : Anti-PECI Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - PECI Antibody (C-term) AW5533
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - PECI Antibody (C-term) AW5533
    PECI Antibody (C-term) (Cat. #AW5533) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession O75521
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=44,40 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 10455
Antigen Region 331-358
Other Names Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3), delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3, 2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPECI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ECI2
Synonyms DRS1, HCA88, PECI
Function Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates (By similarity).
Cellular Location Isoform 1: Mitochondrion.
Tissue Location Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells
Research Areas

BACKGROUND

PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.

REFERENCES

Scanlan,M.J., et.al., Int. J. Cancer 83 (4), 456-464 (1999)
Suk,K., et.al., Biochim. Biophys. Acta 1454 (1), 126-131 (1999)

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