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COX5B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - COX5B Antibody (Center) AW5532
    All lanes : Anti-COX5B Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P10606
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=14;M=14;R=14 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1329
Antigen Region 21-49
Other Names Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B
Dilution WB~~1:1000
Target/Specificity This COX5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the Central region of human COX5B.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOX5B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COX5B
Function This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Cellular Location Mitochondrion inner membrane.
Research Areas

BACKGROUND

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

REFERENCES

Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Chen, Z.X., et al. Cell Death Differ. 17(3):408-420(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Beauchemin, A.M., et al. Brain Res. Bull. 56 (3-4), 285-297 (2001) :
Wu, H., et al. J. Biol. Chem. 275(42):32491-32498(2000)

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