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ATP6V1G1 Antibody(C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ATP6V1G1 Antibody(C-term) AW5529
    All lanes : Anti-ATP6V1G1 Antibody (C-term) at 1:1000 dilution Lane 1: human kidney lysate Lane 2: ZR-75-1 whole cell lysate Lane 3: mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O75348
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=14;M=14 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 9550
Antigen Region 90-118
Other Names V-type proton ATPase subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16, ATP6V1G1, ATP6G, ATP6G1, ATP6J
Dilution WB~~1:1000
Target/Specificity This ATP6V1G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the C-terminal region of human ATP6V1G1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP6V1G1 Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP6V1G1
Synonyms ATP6G, ATP6G1, ATP6J
Function Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Tissue Location Ubiquitous..
Research Areas

BACKGROUND

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized.

REFERENCES

Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Morel, N. Biol. Cell 95(7):453-457(2003)
Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)

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