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EBAG9 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EBAG9 Antibody (Center) AW5528
    All lanes : Anti-EBAG9 Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O00559
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=24,29;M=24;R=24 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 9166
Antigen Region 88-117
Other Names Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG9, RCAS1
Dilution WB~~1:1000
Target/Specificity This EBAG9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human EBAG9.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEBAG9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EBAG9
Synonyms RCAS1
Function May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases.
Cellular Location Golgi apparatus membrane; Single-pass type III membrane protein. Note=According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain
Tissue Location Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs
Research Areas

BACKGROUND

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

REFERENCES

Wolf, J., et al. FASEB J. 24(10):4000-4019(2010)
Sonoda, K., et al. Exp. Cell Res. 316(11):1795-1803(2010)
Ali-Fehmi, R., et al. Cancer Biomark 6(1):33-48(2010)
Yang, Y.C., et al. Zhonghua Wai Ke Za Zhi 47(13):999-1001(2009)
Adamek, D., et al. Folia Neuropathol 47(3):240-246(2009)

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