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ACAT1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ACAT1 Antibody (C-term) AW5517
    All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: mouse liver lysates Lane 3: SK-BR-3 whole cell lysates Lane 4: SW620 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ACAT1 Antibody (C-term) AW5517
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AW5517), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P24752
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=45.2 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 38
Antigen Region 311-349
Other Names Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
Dilution WB~~1:1000
IHC-P~~1:10~50
Target/Specificity This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACAT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAT1
Synonyms ACAT, MAT
Function Plays a major role in ketone body metabolism.
Cellular Location Mitochondrion.
Research Areas

BACKGROUND

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

REFERENCES

Locke,J.A.,Prostate 68 (1), 20-33 (2008)
Guo,Z.Y.,Biochemistry 46 (35), 10063-10071 (2007)
Haapalainen,A.M.,Biochemistry 46 (14), 4305-4321 (2007)

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