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(Mouse) Sox17 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 2 - (Mouse) Sox17 Antibody (C-term) AW5515
    AW5515 staining (Mouse) Sox17 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 3 - (Mouse) Sox17 Antibody (C-term) AW5515
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AW5515 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • 1 - (Mouse) Sox17 Antibody (C-term) AW5515
    All lanes : Anti-Sox17 Antibody (C-term) at 1:2000 dilution Lane 1: F9 whole cell lysates Lane 2: mouse testis lysates Lane 3: LNCap whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q61473
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW M=45,31;H=44;R=45 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 20671
Antigen Region 353-387
Other Names Transcription factor SOX-17, Sox17, Sox-17
Dilution IHC~~1:25
IF~~1:25
WB~~1:2000
Target/Specificity This mouse Sox17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 353-387 amino acids from the C-terminal region of mouse Sox17.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Sox17 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Sox17
Synonyms Sox-17
Function Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'- AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells. Isoform 2 (T-SOX17) shows no DNA-binding activity.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267, ECO:0000269|PubMed:20802155}
Tissue Location Testis. Detected in lung.
Research Areas

BACKGROUND

Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'- AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells. Isoform 2 (T-SOX17) shows no DNA-binding activity.

REFERENCES

Kanai Y.,et al.J. Cell Biol. 133:667-681(1996).
Carninci P.,et al.Science 309:1559-1563(2005).
Layfield R.,et al.Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases.
Kanai-Azuma M.,et al.Development 129:2367-2379(2002).
Kim I.,et al.Cell 130:470-483(2007).

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