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RHOXF2B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RHOXF2B Antibody (Center) AW5505
    All lanes : Anti-RHOXF2B Antibody (Center) at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - RHOXF2B Antibody (Center) AW5505
    Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center)(Cat#AW5505).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF
Primary Accession P0C7M4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB39659
Calculated MW H=32 KDa
Additional info
Gene ID 727940
Other Names Rhox homeobox family member 2B, RHOXF2B
Target/Specificity This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-141 amino acids from the Central region of human RHOXF2B.
Dilution WB~~1:1000
IF~~1:10~50
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRHOXF2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RHOXF2B
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}

BACKGROUND

The function of this protein remains unknown.

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