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(Mouse) Epcam Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - (Mouse) Epcam Antibody (C-term) AW5496
    AW5496 staining Epcam in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - (Mouse) Epcam Antibody (C-term) AW5496
    AW5496 staining Epcam in Human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 4 - (Mouse) Epcam Antibody (C-term) AW5496
    Overlay histogram showing HepG2 cells stained with AW5496 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5496, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - (Mouse) Epcam Antibody (C-term) AW5496
    All lanes : Anti-Epcam Antibody (C-term) at 1:1000 dilution Lane 1: mouse kidney lysates Lane 2: mouse lung lysates Lane 3: mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IHC-P
Primary Accession Q99JW5
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW M=35;H=35;R=35 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 17075
Antigen Region 302-335
Other Names Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Dilution IHC-P~~1:25
FC~~1:25
WB~~1:1000
Target/Specificity This mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Epcam Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Epcam
Synonyms Tacstd1
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein. Lateral cell membrane. Cell junction, tight junction Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction.

BACKGROUND

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

REFERENCES

Bergsagel P.L.,et al.J. Immunol. 148:590-596(1992).
Carninci P.,et al.Science 309:1559-1563(2005).

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