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APRT Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - APRT Antibody (N-term) AW5489
    All lanes : Anti-APRT Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: A2058 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - APRT Antibody (N-term) AW5489
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - APRT Antibody (N-term) AW5489
    Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - APRT Antibody (N-term) AW5489
    Fluorescent image of A549 cell stained with APRT Antibody (N-term)(Cat#AW5489/SA091009AF).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, IHC-P, WB
Primary Accession P07741
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=20,15;M=20;R=20 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 353
Antigen Region 27-60
Other Names Adenine phosphoribosyltransferase, APRT, APRT
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
IF~~1:10~50
Target/Specificity This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human APRT.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPRT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APRT
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

REFERENCES

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

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