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EXOC3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - EXOC3 Antibody (C-term) AW5482
    AW5482 staining EXOC3 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - EXOC3 Antibody (C-term) AW5482
    All lanes : Anti-EXOC3 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: human brain lysates Lane 3: Hela whole cell lysates Lane 4: U-2 OS whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P
Primary Accession O60645
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=87,74,86 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 11336
Antigen Region 524-557
Other Names Exocyst complex component 3, Exocyst complex component Sec6, EXOC3, SEC6, SEC6L1
Dilution IHC-P~~1:25
WB~~1:1000
Target/Specificity This EXOC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 524-557 amino acids from the C-terminal region of human EXOC3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEXOC3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EXOC3
Synonyms SEC6, SEC6L1
Function Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Tissue Location Expressed in epididymis (at protein level).
Research Areas

BACKGROUND

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

REFERENCES

Li J.,et al.Mol. Cell. Proteomics 9:2517-2528(2010).
Jikuya H.,et al.DNA Res. 10:49-57(2003).
Schmutz J.,et al.Nature 431:268-274(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.

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