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GLS Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GLS Antibody (C-term) AW5478
    All lanes : Anti-GLS Antibody (C-term) at 1:1000 dilution Lane 1: mouse brain lysates Lane 2: human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - GLS Antibody (C-term) AW5478
    Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody (C-term)(Cat#AW5478). AW5478 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - GLS Antibody (C-term) AW5478
    Fluorescent image of Hela cells stained with XAF1 GLS Antibody (C-term)(Cat#AW5478). AW5478 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P
Primary Accession O94925
Other Accession P13264, D3Z7P3
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB22875
Calculated MW H=73,65;M=74,66;R=74,66 KDa
Additional info
Gene ID 2744
Other Names Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Target/Specificity This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLS
Synonyms GLS1, KIAA0838
Function Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Cellular Location Isoform 1: Cytoplasm, cytosol.
Tissue Location Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver Isoform 2 is expressed in cardiac and skeletal muscle
Research Areas

BACKGROUND

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

REFERENCES

Swierczynski,J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993)

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