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MICA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MICA Antibody (Center) AW5477
    All lanes : Anti-MICA Antibody (Center) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: Daudi whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - MICA Antibody (Center) AW5477
    Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green, Cat#AP8626C) compared to an isotype control of rabbit IgG(blue). AP8626C was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - MICA Antibody (Center) AW5477
    Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using MICA Antibody (Center)(Cat#AP8626C). AP8626C was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - MICA Antibody (Center) AW5477
    Immunohistochemical analysis of paraffin-embedded H. hepatic carcinoma section using MICA Antibody (Center)(Cat#AP8626C). AP8626C was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC
Primary Accession Q29983
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB21777
Calculated MW H=43,31 KDa
Additional info
Gene ID 100507436
Other Names MHC class I polypeptide-related sequence A, MIC-A, MICA {ECO:0000312|EMBL:CAI419071}
Target/Specificity This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human MICA.
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMICA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MICA {ECO:0000312|EMBL:CAI41907.1}
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Cellular Location Cell membrane; Single- pass type I membrane protein Cytoplasm Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum
Tissue Location Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells
Research Areas

BACKGROUND

MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

REFERENCES

Bahram,S., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6259-6263 (1994) Klein,J.et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (14), 6251-6252 (1994) Parham,P., et.al., J. Immunol. 142 (11), 3937-3950 (1989)

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