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CBFB Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CBFB Antibody (Center) AW5475
    All lanes : Anti-CBFB Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CBFB Antibody (Center) AW5475
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - CBFB Antibody (Center) AW5475
    CBFB Antibody (Center) (Cat. #AW5475) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - CBFB Antibody (Center) AW5475
    Confocal immunofluorescent analysis of CBFB Antibody (Center) (Cat. #AW5475) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, IHC-P, WB
Primary Accession Q13951
Other Accession Q08024
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=22,22 M=22,22,18,1 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 865
Antigen Region 61-90
Other Names Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBFB
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Target/Specificity This CBFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human CBFB.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBFB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBFB
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1.
Cellular Location Nucleus.

BACKGROUND

CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.

REFERENCES

Andersen,C.L., et.al., Br. J. Cancer 100 (3), 511-523 (2009)

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