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SNAP25 Antibody (S187)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SNAP25 Antibody (S187) AW5468
    All lanes : Anti-SNAP25 Antibody (S187) at 1:1000 dilution Lane 1: mouse brain lysates Lane 2: mouse cerebellum lysates Lane 3: human brain lysates Lane 4: human cerebellum lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P60880
Other Accession P60881, P60879, P60878, Q17QQ3, Q5TZ66
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=23;M=23;R=23 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 6616
Antigen Region 179-213
Other Names Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP
Dilution WB~~1:1000
Target/Specificity This SNAP25 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 179-213 amino acids from the region of human SNAP25.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNAP25 Antibody (S187) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNAP25
Synonyms SNAP
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Cellular Location Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity).
Tissue Location Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum
Research Areas

BACKGROUND

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

REFERENCES

Bark I.C.,et al.Gene 139:291-292(1994).
Zhao N.,et al.Gene 145:313-314(1994).
Jagadish M.N.,et al.Biochem. J. 317:945-954(1996).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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