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NDUFA12 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NDUFA12 Antibody (N-term) AW5465
    All lanes : Anti-NDUFA12 Antibody (N-term) at 1:1000 dilution Lane 1: human skeletal muscle lysates Lane 2: HepG2 whole cell lysates Lane 3: HT-1080 whole cell lysates Lane 4: mouse liver lysates Lane 5: mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9UI09
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB50815
Calculated MW H=17,7;M=17 KDa
Additional info
Gene ID 55967
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B172, CI-B172, CIB172, NADH-ubiquinone oxidoreductase subunit B172, NDUFA12, DAP13
Target/Specificity This NDUFA12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 34-68 amino acids from the N-terminal region of human NDUFA12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFA12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFA12
Synonyms DAP13
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas

BACKGROUND

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

REFERENCES

Triepels R.,et al.Hum. Genet. 106:385-391(2000).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).
Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003).

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