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HIST1H2BM Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HIST1H2BM Antibody (Center) AW5461
    All lanes : Anti-HIST1H2BM Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysates Lane 2: PC-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q99879
Other Accession P02283, P57053, Q9PSW9, P0C1H5, P0C1H4, Q6PC60, Q8CGP0, Q8N257, Q9D2U9, Q5QNW6, Q64524, Q16778, Q64525, Q00715, Q5BJA5, P0C1H3, P62808, Q8CGP2, P23527, Q99877, Q32L48, P10854, Q99880, Q8CGP1, O60814, Q2M2T1, P06899, Q64478, Q93079, P10853, P58876, Q6ZWY9
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=14;M=14 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 8342
Antigen Region 44-78
Other Names Histone H2B type 1-M, Histone H2Be, H2B/e, HIST1H2BM, H2BFE
Dilution WB~~1:1000
Target/Specificity This HIST1H2BM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-78 amino acids from the Central region of human HIST1H2BM.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H2BM Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H2BM
Synonyms H2BFE
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.

BACKGROUND

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

REFERENCES

Albig W.,et al.Hum. Genet. 101:284-294(1997).
Marzluff W.F.,et al.Genomics 80:487-498(2002).
Mungall A.J.,et al.Nature 425:805-811(2003).
Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
Cheung W.L.,et al.Cell 113:507-517(2003).

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