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CHRM2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 4 - CHRM2 Antibody AW5457
    Overlay histogram showing SH-SY5Y cells stained with (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CHRM2 Antibody AW5457
    All lanes : Anti-CHRM2 Antibody at 1:1000 dilution Lane 1: human brain lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: U-87 MG whole cell lysates Lane 4: Y79 whole cell lysates Lane 5: Neuro-2a whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - CHRM2 Antibody AW5457
    Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody (Cat#AW5457 ). AW5457 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
  • 14 - CHRM2 Antibody AW5457
    Immunohistochemical analysis of paraffin-embedded H. brain section using CHRM2(Cat#AW5457 ). AW5457 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - CHRM2 Antibody AW5457
    Immunohistochemical analysis of paraffin-embedded H. heart section using CHRM2 (Cat#AW5457 ). AW5457 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P08172
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1424CT461.78.60
Calculated MW H=52;M=52 KDa
Additional info
Gene ID 1129
Other Names Muscarinic acetylcholine receptor M2, CHRM2
Target/Specificity This antibody is generated from a mouse immunized with a recombinant protein.
Dilution FC~~1:25
WB~~1:1000
IF~~1:25
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHRM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHRM2
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Phosphorylation in response to agonist binding promotes receptor internalization.
Research Areas

BACKGROUND

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

REFERENCES

Bonner T.I.,et al.Science 237:527-532(1987).
Peralta E.G.,et al.EMBO J. 6:3923-3929(1987).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Kitano T.,et al.Mol. Biol. Evol. 21:936-944(2004).
Gurevich V.V.,et al.J. Biol. Chem. 270:720-731(1995).

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