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HAO1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HAO1 Antibody (Center) AW5452
    All lanes : Anti-HAO1 Antibody (Center) at 1:1000 dilution Lane 1: human liver lysates Lane 2: mouse liver lysates Lane 3: rat liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9UJM8
Other Accession Q9WU19
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=41;M=41;R=41 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 54363
Antigen Region 157-185
Other Names Hydroxyacid oxidase 1, HAOX1, Glycolate oxidase, GOX, HAO1, GOX1, HAOX1
Dilution WB~~1:1000
Target/Specificity This HAO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-185 amino acids from the Central region of human HAO1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHAO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAO1
Synonyms GOX1, HAOX1
Function Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate.
Cellular Location Peroxisome.
Tissue Location Liver.
Research Areas

BACKGROUND

HAO1 is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids.

REFERENCES

Jones,J.M., et.al., J. Biol. Chem. 275 (17), 12590-12597 (2000)
Kohler,S.A., et.al., J. Biol. Chem. 274 (4), 2401-2407 (1999)

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