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MEK1 (MAP2K1) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MEK1 (MAP2K1) Antibody (N-term) AW5451
    All lanes : Anti-MEK1 Antibody (M1) at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: C6 whole cell lysates Lane 3: PC-12 whole cell lysates Lane 4: mouse brain lysates Lane 5: human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q02750
Other Accession Q01986, P29678, P31938
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=43;M=43;R=43 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5604
Antigen Region 1-30
Other Names Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1
Dilution WB~~1:1000
Target/Specificity This MEK1 (MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK1 (MAP2K1).
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMEK1 (MAP2K1) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K1
Synonyms MEK1, PRKMK1
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis
Tissue Location Widely expressed, with extremely low levels in brain.
Research Areas

BACKGROUND

MEK1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

REFERENCES

Zheng, B., et al., Blood 102(3):1019-1027 (2003).
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Zhu, X., et al., J. Neurochem. 86(1):136-142 (2003).
Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003).
Witowsky, J.A., et al., J. Biol. Chem. 278(3):1403-1406 (2003).

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