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ROR1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ROR1 Antibody AW5449
    All lanes : Anti-ROR1 Antibody at 1:1000 dilution Lane 1: human lung lysates Lane 2: K562 whole cell lysates Lane 3: mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ROR1 Antibody AW5449
    Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody (Cat#AW5449). AW5449 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 4 - ROR1 Antibody AW5449
    Flow cytometric analysis of A549 cells using ROR1 Antibody (green, Cat#AP7671d) compared to an isotype control of rabbit IgG(blue). AP7671d was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession Q01973
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=104,43,44 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4919
Antigen Region 112-399
Other Names Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Dilution WB~~1:1000
IHC-P~~1:25
FC~~1:25
Target/Specificity This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsROR1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ROR1
Synonyms NTRKR1
Function Tyrosine-protein kinase receptor whose role is not yet clear.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm
Research Areas

BACKGROUND

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

REFERENCES

Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).

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