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TPI1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TPI1 Antibody (C-term) AW5440
    All lanes : Anti-TPI1 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: LNCaP whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - TPI1 Antibody (C-term) AW5440
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - TPI1 Antibody (C-term) AW5440
    TPI1 Antibody (C-term) (Cat. #AW5440) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P60174
Other Accession P48500, P00939, P17751, Q60HC9, Q5E956
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=31,27,18;M=32;R=27 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 7167
Antigen Region 258-286
Other Names Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPI1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPI1
Synonyms TPI
Research Areas

BACKGROUND

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

REFERENCES

Martins-de-Souza,D., et.al., BMC Psychiatry 9, 17 (2009)

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